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Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 97 RPC R 58 - Programme Pathologie - Rapport Scientifique année 1997 ArchiMer
Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Cochennec, Nathalie.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00043/15454/12829.pdf
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Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 98 RPC R 7 - Programme Pathologie - Rapport Final année 1998 ArchiMer
Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En...
Tipo: Text Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf
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Contribution à la mise en place de critères de qualité des huîtres creuses Crassostrea gigas affinées en claires ArchiMer
Gras, Marie-paule; Gras, Paul; Cosnard, Michel; Garnier, Jacqueline.
The oysters fattened in the "claires" have a very appreciated quality. Representative criters of this quality: index of condition, the glycogen content, color of gills of molluscs by the pigment called "marennine" and indx of shape of shells are studied for the Japanses oysters Crassostrea gigas (Thunberg)
Tipo: Text Palavras-chave: Ostréiculture; Claires à huître; Crassostrea gigas; Marennine; Verdissement; Marennes Oléron; Charente Maritime.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00009/12009/8701.pdf
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Contribution à l'étude de l'ontogenèse du système immunitaire chez l'huître creuse Crassostrea gigas ArchiMer
Tirape Bajana, Ana.
The interest for the understanding of the immune mechanisms in the oyster, Crassotrea gigas has been considerably increased these last years, mainly because of economie losses due to diseases which affected the continuity of this species. The studies of these mechanisms contributes to a better understanding of the differential susceptibilities observed in this species against various pathogens. In the present study, our  goal was to define the ontogenesis of immune system in C. gigas during the development, by functional genomic approaches. The studies have been focalized on the analyses of expression and localization of effectors previously identified in adult oysters as implicated in immunity. We have shown, in particular, that hemocytes appear in the...
Tipo: Text Palavras-chave: Invertébré; Mollusque bivalve; Huître; Cra; Immunité; Hémocytes; Hématopoïèse; Développement; Ontogenèse; Expression des gènes; Q-PCR; SSH; Invertebrate; Bivalve Mollusk; Oyster; Crassostrea gigas; Immunity; Hemocytes; Hematopoiesis; Development; Ontogenesis; Gene expression; Q-PCR; SSH.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00440/55113/56565.pdf
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Contribution à l'étude de mollusques marins : Iridovirus-like et Herpèsvirus-like. Description, caractérisation biochimique, cycle de multiplication viral, diagnostic et étude épidémiologique ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Among the marine molluscs, Pacifie oyster, Crassostrea gigas, is actually the most reared specie in the world. Noticely in France, where it was imported between 1960 and 1970, and replaced by Portuguese oyster, Crassostrea angulata, which specie was decimated during this period because of iridoviruses. Uncontroled introductions of new species may however be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases of bivalve molluscs in importing country. In addition to these observations, the quasi monobreeding of Pacific oyster in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this specie, namely the diagnosis of viral diseases. Several families of viruses...
Tipo: Text Palavras-chave: Mollusques; Crassostrea gigas; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Molluscs; Crassostrea gigas; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00041/15230/12548.pdf
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Contribution à l'étude de vibrions pathogènes d'huîtres creuses Crassostrea gigas - Interactions hémocytes-vibrions et développement de tests de diagnostic d'intérêt taxonomique ArchiMer
Duperthuy, Marylise.
Les bactéries du genre Vibrio représentent le groupe le plus fréquemment associé à des maladies infectieuses chez les mollusques marins et notamment l'huître creuse Crassostrea gigas. A ce jour trois espèces de Vibrio pathogènes d'huîtres creuses V. splendidus, V. aestuarianus et récemment V. coralliitycus ont été identifiés en France. La prévention des mortalités associées à ces vibrioses repose sur une meilleure compréhension des interactions hôte-pathogène et sur l'utilisation de techniques de diagnostic rapides à mettre en oeuvre et fiables. Ce travail a permis d'une part de caractériser la dynamique de phagocytose de vibrions par les cellules immunocompétentes de l'huître, les hémocytes. La mise au point d'un protocole d'analyse en cytométrie de flux...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Vibrio splendidus; Vibrio aestuarianus; Vibrio corralliitycus; Hémocytes; Phagocytose; Cytométrie de flux; Diagnostic; PCRq.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00033/14452/11753.pdf
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Contribution à l'étude de virus de mollusques marins apparentés aux Iridoviridae et aux Herpesviridae ArchiMer
Le Deuff, Rose-marie.
Among the marine molluscs, Pacific oyster, Crassostrea gigas, is actually the most reared species in the world. Noticeably in France, where it was imported between 1960 and 1970, and replaced Portuguese oyster, Crassostrea angulata. Intensification of breeding and the associated introductions of new species may be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases in bivalve molluscs. In addition to these observations, the quasi monobreeding of C. gigas in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this species, namely the diagnosis of viral diseases. Several families of viruses have been described within marine bivalves molluscs. The purpose of this...
Tipo: Text Palavras-chave: Mollusques bivalves; Crassostrea gigas; Huître japonaise; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Bivalve molluscs; Crassostrea gigas; Pacific oyster; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00107/21792/19367.pdf
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Contribution à l'étude du stockage des huîtres en bassins ArchiMer
Masson, Daniel.
La mise sur le marché de coquillages marins vivants nécessite dans la majorité des cas un stockage en bassin avant expédition. Cette opération a plusieurs buts : - les débarrasser de la vase ou du sable qu'ils ont pu accumuler dans leur cavité palléale, sur les zones où ils vivaient, - favoriser l'élimination de germes éventuels (dont certains sont pathogènes pour l'homme) par le biais de l'activité de filtration des coquillages eux-mêmes, - permettre l'activité respiratoire de ces coquillages avant une période d'émersion plus ou moins longue (conditionnement, transport, mise en vente). Le coquillage conditionné et expédié est une denrée alimentaire. Avant cela (et notamment lors du stockage) il doit être considéré comme un animal d'élevage et traité en...
Tipo: Text Palavras-chave: Ostréiculture; Huître creuse; Crassostrea gigas; Stockage en bassin; Expérimentations; Physiologie.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00134/24496/22524.pdf
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Contribution du Laboratoire Conchylicole de Poitou Charente (LCPC) au programme national sur les MORtalités ESTivales (MOREST) de l'huître creuse Crassostrea gigas. Résumé des principales actions menées en 2001 ArchiMer
Soletchnik, Patrick.
Au cours de l’année 2001, dans le cadre de la première année du programme national Morest sur les mortalités estivales d’huîtres creuses, Crassostrea gigas, le laboratoire conchylicole du Poitou-Charentes a été amené à travailler sur plusieurs opérations : les différents types de mortalité, leur caractérisation sur les trois sites ateliers du programme (Baie des Veys, rivière d’Auray et banc de Perquis dans le sud du bassin de Marennes), la caractérisation des familles génétiques produites en écloserie et la cinétique de mortalité sur le site de Fouras : secteur de captage traditionnel dans le bassin de Marennes. Enfin, des tests ont été réalisés sur les naissains produits en écloserie afin de tenter des trouver un indicateur physiologique de leur...
Tipo: Text Palavras-chave: Mortalité estivale; Huîtres creuses; Crassostrea gigas; Bassin de Marennes Oléron; MOREST.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00164/27548/25713.pdf
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Contribution of Viral Genomic Diversity to Oyster Susceptibility in the Pacific Oyster Mortality Syndrome ArchiMer
Delmotte, Jean; Chaparro, Cristian; Galinier, Richard; De Lorgeril, Julien; Petton, Bruno; Stenger, Pierre-louis; Vidal-dupiol, Jeremie; Destoumieux-garzon, Delphine; Gueguen, Yannick; Montagnani, Caroline; Escoubas, Jean Michel; Mitta, Guillaume.
Juvenile Pacific oysters (Crassostrea gigas) are subjected to recurrent episodes of mass mortalities that constitute a threat for the oyster industry. This mortality syndrome named “Pacific Oyster Mortality Syndrome” (POMS) is a polymicrobial disease whose pathogenesis is initiated by a primary infection by a variant of an Ostreid herpes virus named OsHV-1 μVar. The characterization of the OsHV-1 genome during different disease outbreaks occurring in different geographic areas has revealed the existence of a genomic diversity for OsHV-1 μVar. However, the biological significance of this diversity is still poorly understood. To go further in understanding the consequences of OsHV-1 diversity on POMS, we challenged five biparental families of oysters to two...
Tipo: Text Palavras-chave: Crassostrea gigas; Herpesvirus diversity; Genotype-genotype interactions; Oyster genetic background; Viral populations.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00643/75533/76414.pdf
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Contributions du Laboratoire Conchylicole de Normandie (LCN) au défi MOREST en 2002 ArchiMer
Ropert, Michel; Simonne, Charlotte; Hugonnet, Virginie; Le Gagneur, Eric; Kopp, Joel.
This report present results obtained by the LCN in coastal Ecology of the MOREST program. Most of works presented here have been realised in collaboration with different partners who are cited before each action. This report is divided in 8 parts in 3 themes. WP2 : Characterisation of in-situ summers mortalities • Task 2.2.1. : dynamics of mortality, reproduction and spawning. lntersite comparisons (BASE) • Task 2.2.4 : study of the summer mortalities dynamics of Crassostrea gigas in Bay of Veys (SUMO) WP4 : Experimental tests to cast off summer mortalities problem • Task 4.3.: reduce the critical period before laid: induce oyster laid in the Bay of Veys. • Task 4.4.1.: effects of transfer livestock on summer mortalities : move oysters before the...
Tipo: Text Palavras-chave: Crassostrea gigas; Mortalité; MOREST; Baie de Veys; Normandie; Crassostrea gigas; Mortality; MOREST; Bay of Veys; Normandy.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00436/54780/56238.pdf
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Contributions du Laboratoire Conchylicole de Poitou-Charente au défi MOREST en 2002. Thématique "Ecologie côtière" ArchiMer
Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Guilpain, Patrice; Geairon, Philippe; Razet, Daniel; Madec, Patrick; Robert, Stephane; Taillade, Sylvie; Doner, Anne.
This report presents results obtained by the LCPC in coastal Ecology. The contributions of the LCPC, as partner associated with the genetic studies, do not appear in this report. This report is divided in 5 parts of differing importance : • Task 2.2.3. A "multi-disciplinary study of the DYNAmics of estival mortalities of Crassostrea gigas in the Marenne - Oléron Basin (Dynamo-2002)"; study developed and directed by the LCPC after the “plat-table” C. gigas mortality model in the Marennes- Oleron Basin. • Task 2.3 . "Influence of autumn and winter conditions on the maturation of parents (phase 1) and on the survival of their offspring (phase 2) (Gegen). Action directed by the LCPC. • Task 2.2.1. " Dynamics of mortality, reproduction and spawning....
Tipo: Text Palavras-chave: MOREST; Crassostrea gigas; Mortalité; Ecologie côtière; MOREST; Crassostrea gigas; Mortality; Coastal Ecology.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00164/27549/25714.pdf
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Contributions du Laboratoire Conchylicole de Poitou-Charente (LCPC) au défi MOREST (mortalités estivales d'huîtres creuses) en 2003. Thématique "Ecologie côtière" ArchiMer
Soletchnik, Patrick; Le Moine, Olivier; Geairon, Philippe; Razet, Daniel; Blouin, Frederic; David, Delphine; Couty, Angelique.
Depuis 2001, le laboratoire participe au défi MOREST, comme laboratoire d'Ecologie côtière dans l'organisation et l'animation d'études pluridisciplinaires et comme site atelier du défi. En 2003, le LCPC encadre une opération de recherche impliquant 14 laboratoires sur l'étude du modèle de mortalité plat table dans le Bassin de Marennes Oléron. Un large panel des causes de mortalité est étudié; facteurs physico chimiques, agents pathogènes, substances toxiques (pesticides ou relargages chimiques), etc …. Le labo est également impliqué en accompagnement d'actions de caractérisation de la mortalité de naissain (lignées consanguines sur estran) et sur des opérations de caractérisation précoce de la mortalité en laboratoire. Enfin, l'effet "site de gestion...
Tipo: Text Palavras-chave: Mortalité estivale; Huîtres creuses; Crassostrea gigas; Bassin de Marennes Oléron; MOREST.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00164/27550/25715.pdf
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Contrôle de la gamétogénèse des huîtres creuses et plates. Relations "reproduction" et "génétique" ArchiMer
Gerard, Andre; Naciri-graven, Yamama; Boudry, Pierre; Launey, Sophie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal.
At the end of the 1980s, after two decades of aquaculture research in molluscs, Ifremer initiated its first genetic studies. These new studies quickly revealed the limits of knowledge in aquaculture, particularly in terms of controlling oyster reproduction. This presentation attempts to summarize the available information on genetics in aquaculture. In particular, breeding practices in the 1980s involved populations of broodstock, while genetics requires modern techniques where individuals, not just populations, can be identified. Controlling reproduction at the individual level in oysters raises new questions that existing protocols cannot address, especially in terms of controlling sex determination, individual maturity, spawning synchrony, chemical...
Tipo: Text Palavras-chave: Gamètes; Fécondation; Induction ponte; Reproducteur; Conditionnement; Gamétogénèse; Génétique; Reproduction; Crassostrea gigas; Huîtres.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/1995/acte-3210.pdf
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Contrôle des performances biologiques de populations diploïdes et triploïdes d'huîtres creuses Crassostrea gigas ArchiMer
Benhaim, David.
Un programme concerannt le contrôle des perforùances biologiques de lots diploïdes et triploïdes d'huîtres creuses Crassostrea gigas a débuté en mars 1993 à La Tremblade et dans d'autres station IFREMER associées. Le présent rapport rend compte des cinq premiers mois d'analyses faisant partie d'un contrôle qui devrait se poursuivre jusqu'en 1994. A La Tremblade, l'expéreince repose principalement sur la comparaison des paramètres biométriques et biochimiques des lots diploïdes et triploïdes en deux sites d'étude : un parc ostréicole sur le bassin de Marennes-Oléron et une claire. Les individus diploïdes réfractaires du lot triploïde, sont identifiés par imagerie numérique et écartés des analyses. Les résultats obtenus montrent une difféence marquée entre...
Tipo: Text Palavras-chave: Crassostrea gigas; Triploïdes; Diploïde; Estran; Croissance; Biochimie.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00411/52220/52948.pdf
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Croissance de l'huître creuse Crassostrea gigas dans le bassin de Marennes-Oléron de 1987 à 1998 ArchiMer
Le Moine, Olivier; Geairon, Philippe.
This report aims to study the oyster Crassostrea gigas growth and production over a 12 years time's span in the Bay of Marennes Oleron. Results of 12 years of oyster growth did not show any significant oyster biological productivity decrease, nor trend. In contrast, annual growth showed a large variability, which will be correlated with environmental conditions. Moreover, annual growth variability was greater than the variability due to site location, but the Ronce experimental site. The later, located in the south west part of the Bay of Marennes Oleron, has been the leading oyster growth site since 1989. The annual shell growth variability was greater than oyster dry meat weight fluctuation. Both 1990 and 1991 years were characterized by the largest...
Tipo: Text Palavras-chave: Marennes Oleron bay; Crassostrea gigas; Monitoring network; Oyster growth and production; Marennes Oléron; Crassostrea gigas; Réseau de surveillance; Production; Croissance.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/rapport-1588.pdf
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Croissance de l'huitre japonaise Crassostrea gigas en cylindre rotatif Stanway. Premiers resultats en France ArchiMer
Robert, Rene; Maurer, Daniele.
A new method of oyster cultivation, the Stanway oyster cylinder, has been experimented in the basin of Arcachon since the autumn of 1989. The first observations on three species of juvenile bivalves showed that this system could be successfully used with the Japanese oyster Crassostrea gigas . A comparative study of the growth of this last species, reared in cylinders and oyster bags, has been carried out since spring 1990. The first results showed that the growth of juveniles and 18 months old oysters in cylinders is not as good as in bags. On the other hand, mortality did not differ between both techniques: less than 3%. This new system seems to be less efficient than the traditional French way of rearing oysters. However, an improvement in the quality...
Tipo: Text Palavras-chave: Crassostrea gigas; Bivalvia; Juveniles; Oyster culture; Growth; Rearing.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-917.pdf
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Croissance des huîtres creuses, Crassostrea gigas, en baie du mont Saint-Michel, avant la restructuration de 2004 ArchiMer
Bouget, Jean-francois; Mazurie, Joseph.
This study presents the main results of oyster growth obtained experimentally in 2002 and 2003, in the farming area of Cancale before the leases reorganization of 2004. According to the REMORA monitoring network from 1990 to 2004, the oyster growth in Cancale bay may be characterized by an average annual weight gain (around 30g per year), a rather weak meat content compared to other regional oyster sites, and an exceptional quality of shell (absence of blisters, few Poly do rd). Spatially, the oyster farming area of Cancale shows rather homogeneous performances of breeding, in spite of the muddy southern area. In addition an experimental trial in 2003 allowed to evaluate favourably the potential of the new oyster area of Saint-Benoît : in these...
Tipo: Text Palavras-chave: Mont Saint Michel; Crassostrea gigas; Growth; Breeding; Oyster; Mont Saint Michel; Crassostrea gigas; Croissance; Ostréiculture; Huître.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/rapport-1637.pdf
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Croissance des larves de Crassostrea gigas et de Mytilus galloprovincialis en présence d'algues monocellulaires isolées du tractus digestif des véligères du milieu naturel ArchiMer
His, Edouard; Robert, Rene.
The collection of Crassostrea gigas veligeres in their natural environment and the homogenate technique (HIS et al., 1985) enable the monospecific culture of nine algae which larvae feed on. Four of them could be mass-produced: Nannochloris atomus, Stichococcus bacillaris, Chlamidomonas bullosa and Tetraselmis Tetrathele. Their feeding quality has been tested using the cross-growth method, and using Crassostrea gigas and Mytilus galloprovincialis veligeres. Although regularly found in the homogenates, and thus fed on by larvae, the first three algae gave poor results. Only Tetraselmis tetrathele has good nutritional qualities for Crassostrea gigas veligeres.
Tipo: Text Palavras-chave: Tetraselmis tetrathele; Chlamydomonas bullosa; Stichococcus bacillaris; Nannochloris atomus; Algues unicellulaires; Mytilus galloprovincialis; Crassostrea gigas; Nutrition; Larves.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/acte-6186.PDF
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Croissance, engraissement et cycle sexuel de Crassostrea gigas dans le Bassin d'Arachon: comparaison des huîtres agées de 1 et 2 ans ArchiMer
Maurer, Daniele; Borel, Michel.
Two-year-old oysters have a physiology which is reproduction-oriented, their maturation occurring synchronously among oysters from the same batch. The importance of the spring accumulation of glycogenated stocks varies through the years and correlates with the growth stage. Glucidic components are quickly used during the month preceding egg-laying and are transformed into lipid stocks for the oocytes. One-year-old oysters develop at different rates, some of them not showing any sign of maturation and the percentage of laying individuals varies through the years. Glycogenated stocks accumulate in a similar way in both age-classes and their more precocious and more intense use by young molluscs is not necessarily linked with their gametogenesis and growth,...
Tipo: Text Palavras-chave: Bay of Arcachon; Maturation; Biochemistry; Crassostrea gigas; Bassin d'Arcachon; Maturation; Biochimie; Crassostrea gigas.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/acte-6182.PDF
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